affinity fplc (fast protein liquid chromatography) (Amersham Life Sciences Inc)

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Amersham Life Sciences Inc affinity fplc (fast protein liquid chromatography)
Affinity Fplc (Fast Protein Liquid Chromatography), supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/affinity+fplc+(fast+protein+liquid+chromatography)/affinity+fplc++fast+protein+liquid+chromatography+/pm27676238-112-6-12
Average 90 stars, based on 1 article reviews

affinity fplc (fast protein liquid chromatography) - by Bioz Stars, 2026-10

90/100 stars

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Recombinant:

Article Title: Oxidative DNA Damage and Repair in the Radioresistant Archaeon Thermococcus gammatolerans.
Article Snippet: The soluble proteins were loaded onto His-trap columns (Macherey Nagel) previously equilibrated with buffer A and washed in the same buffer. .. The recombinant proteins were purified by affinity FPLC (Fast Protein Liquid Chromatography) (Amersham, GE Healthcare) with increasing steps of imidazole (50 mM, 150 mM, 250 mM) in the same buffer and dialyzed twice (Spectrumlabs, MWCO 15 kDa) overnight at 4°C against 500 mL buffer B (20 mM Tris-HCl pH 7.5, 50% Glycerol v/v, 200 mM NaCl, 1 mM DTT). ..

Purification:

Fast Protein Liquid Chromatography:

other:

Article Title: Purification and characterization of an alpha-2-macroglobulin protease inhibitor from plasma of the grouper Epinephelus coioides
Article Snippet: Article history: An alpha-2-macroglobulin Received 13 March 2008 Received in revised form 15 July 2008 Accepted 21 July 2008

Article Title: Plesiomonas shigelloides exports a lethal cytotoxic-enterotoxin (LCE) by membrane vesicles
Article Snippet: The concentrates were submitted to Cu 2+ ion metallic affinity chromatography (IMAC) gel packed chromatography column (AKTA FPLC, GE-Amersham Healthcare), equilibrated with 50 mM Tris–HCl buffer, pH 8.0, containing 1 M NaCl, eluted with a 0–0.5 mM linear imidazole gradient.

Article Title: Purification, characterization and molecular cloning of alpha-2-macroglobulin in cobia, Rachycentron canadum.
Article Snippet: Alpha-2-macroglobulin (a-2-M) is a broad spectrum protease inhibitor which is abundant in the plasma of vertebrates and several invertebrates.. The a-2-M was purified from cobia (Rachycentron canadum) plasma by a four-step procedure: poly ethylene glycol fractionation, affinity chromatography, hydrophobic interaction chromatography and ion exchange chromatography on Fast Protein liquid chromatography system in the present study.. It migrated as one protein band with a molecular mass of about 360 kDa in the native state, whereas in SDS-PAGE it was about 180 kDa under non-reducing condition.

Article Title: Effects of high light on transcripts of stress-associated genes for the cyanobacteria Synechocystis sp. PCC 6803 and Prochlorococcus MED4 and MIT9313.
Article Snippet: Received 8 January 2004 Accepted 13 January 2004 Cyanobacteria constitute an ancient, diverse and ecologically important bacterial group.. The responses of these organisms to light and nutrient conditions are finely controlled, enabling the cells to survive a range of environmental conditions.. In particular, it is important to understand how cyanobacteria acclimate to the absorption of excess excitation energy and how stress-associated transcripts accumulate following transfer of cells from lowto high-intensity light.

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